Gene Editing - InVivo Biosystems

Review: How a Small Worm is Helping Uncover the Pathogenicity of Gene Variants

Disease Modeling, Gene Editing, News & Announcements / By Ben Jussila and Alexandra Narin / August 25, 2022 September 2, 2022

We have previously discussed how the advancement of genomics technology has led to an increase in variants of unknown significance (VUS), and how important it is to identify whether or not they are disease-causing. In this review we discuss a recent publication from Dr. Oliver Blacque and collaborators (Lange et al., 2022), where they leveraged …

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Differences Between HDR vs NHEJ

CRISPR, Gene Editing, News & Announcements / By Chesley Johnson / April 19, 2022 April 19, 2022

Summary The CRISPR-Cas9 system has become integral in the toolbelts of genomic scientists, researchers, students, etc. Never has it been so easy to wield a pair of molecular scissors in your (pipetting) hand, however, there are a few intracellular processes to consider before cutting up your genome of interest. In this article, we will give …

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How do Knockins Work?

CRISPR, Gene Editing, View from the Bench / By Karl Stuntzner-Gibson / March 24, 2022 April 8, 2022

In this article we compare and contrast several knock-in methodologies. So how do knock-ins work? Continue reading to find out!

The ABCs of Genetic Research

CRISPR, Gene Editing / By Alexandra Narin / March 22, 2022 March 22, 2022

Here at InVivo Biosystems we spend a lot of time thinking, and talking, about genome editing. We thought it would be fun to compile a list of our frequently used terms – so here they are, our ABCs of genetic research.

Does everyone have digestive issues? — Gut dysbiosis is on the rise.

Disease Modeling, Gene Editing, Nutraceuticals / By Alexandra Narin / March 8, 2022 September 29, 2022

In this article we discuss the rise in gut dysbiosis, and new risk factors: microplastics, consumption of supplements, and chronic stress.

How does Cre/loxP recombination work?

Gene Editing, View from the Bench / By Ashwin Suresh and Jenn Lawson / March 3, 2022 March 7, 2022

In this blog, we discuss the basics of the Cre/Lox technique which is used in conditionally controlling gene manipulation in zebrafish.

What is Zebrafish Knock-in Tagging?

CRISPR, Gene Editing / By Ben Jussila / January 20, 2022 January 20, 2022

With knock-in tagging, you can tag your favorite gene at the native locus in your zebrafish line via CRISPR/Cas9 gene editing technology.

The State of Knock-ins in Zebrafish

CRISPR, Gene Editing / By Alexandra Narin / October 11, 2021 October 12, 2021

The recent advancements of CRISPR and next-generation technology has enabled researchers to create more precise zebrafish models of human disease. This being said, knock-in (KI) techniques in zebrafish still aren’t fully optimized. In this article we discuss the current state of CRISPR-Cas9-mediated targeted knock-ins in zebrafish, and what the future holds.

RedEfish: Generation of the Polycistronic mScarlet: GSG-T2A: Ttpa Zebrafish Line

Gene Editing / By Alexandra Narin / September 23, 2021 April 25, 2022

Dr. Robyn Tanguay is a distinguished professor at Oregon State University, and a collaborator with InVivio Biosystems. Dr. Tangauy utilizes zebrafish to study toxicology, and last month received a $7 million grant from the National Institute of Environmental Health Sciences to study the biological impacts of chemicals. In a recent publication, Dr. Tanguay and her lab created a new fluorescent-tagged zebrafish line to study the effects of Vitamin E on embryonic development. This article will give an overview of the Tanguay Lab’s work, and discuss: ‘why zebrafish’?

A Spotlight on Recent Zebrafish Research

CRISPR, Gene Editing, View from the Bench / By Ben Jussila / November 3, 2020 November 9, 2020

We’ve compiled some of the most recent informative and influential research being done using the zebrafish model