In this blog, we discuss the basics of the Cre/Lox technique which is used in conditionally controlling gene manipulation in zebrafish.
The Undiagnosed Diseases Network UND is a nationwide network that is working to understand and diagnose rare and undiagnosed human diseases. The UND coordinates between research and clinical experts, using the latest genetic and genomic technologies to create a more personalized medicine model.
The Undiagnosed Diseases Network brings together experts across the USA, combining clinical care and research to find diagnoses for undiagnosed patients. Zebrafish are extremely valuable disease models in this research, as they reduce the cost and speed up the process of identifying therapeutic targets. In this blog we highlight one rare disease whose mechanism was successfully identified using zebrafish.
Plasmids are a key tool in molecular biology. These circular pieces of DNA, also called “vectors” or “constructs”, can be engineered to contain large made-to-order stretches of synthetic DNA. We design plasmids when we need to insert large stretches of synthetic DNA into the genome to create a model organism expressing, for instance, a fluorescent …