With the advent of the molecular biology revolution and the human genome project in the 1990s there was a historic shift toward target-based drug development. Target-based drug development, or TDD, relies on prior knowledge of disease mechanisms and targets before development can begin.
We created over 90 point mutations in the STXBP1 gene via CRISPR. A map of these point mutations can be seen above. Clinical variants were selected from the ClinVar database, literature, the Gnomad database, clinical researchers, and the STXBP1 foundation.
June is Alzheimer’s & Brain Awareness Month. We wanted to take a few moments to spotlight some of the awe-inspiring research on Alzheimer’s disease taking place in alternative animal models: C. elegans: Kumar DK, Choi SH, Washicosky KJ, et al. Amyloid-β peptide protects against microbial infection in mouse and worm models of Alzheimer’s disease. Sci …
You may or may not know that at InVivo Biosystems, we conduct a lot of grant funded research as well as offering custom models and assays to our clients. Much of our grant activity in the past few years has centered on creating and characterizing disease-associated variants and point mutations. We’ve created collaborations and partnerships …
We Used CRISPR to Make 15 Point Mutations in 5 Genes. Here’s What We Learned. Read More »
New NIH Grant awarded to InVivo Biosystems to address critical needs in precision medicine research using the model of C. elegans. The grant, titled “Functional Assessment of Variants in Organisms of Research (FAVOR) – Profiling Canonical Human Genes and their Variants through Disease Model Phenotyping” in the amount of $350,000 is awared by the Nation Human Genome Research Institute (NHGRI).
Summary: As the zebrafish is increasingly adopted to test drug toxicity, it is critical to systematically assess the parallels between zebrafish, humans and other model systems. In this article, we highlight a recent paper that compares toxicity results obtained in zebrafish and rats. The paper concludes that zebrafish are “valuable for predicting mammalian toxic exposure …
Zebrafish Acute Toxicity Study Demonstrates Concordance with Rat Fetal Embryotoxicity Read More »
We take a look into why so many zebrafish groups struggle to achieve knock-in transgenesis and what makes knock-ins so difficult.
