C. elegans Behavior & Phenotyping
Defecation
Defecation is an highly regulated ultradian rhythmic process. It can be used to assess defects in neuromuscular signaling.
Defecation Data. pBoc-pBoc (sec)= defecation cycle time between two posterior body contraction. % Exp skipped=no exp/total pBoC (in 10 min).
APPLICATIONS
Can be used to study diseases such as Epilepsy, Alzheimer disease and Parkinson’s disease.
ASSAY DESCRIPTION
Pick one young adult worm to a fresh NGM plate seeded with OP50. Wait 5 min (Worm will not feed or defecate for 1-2 min because of transferring.) and take a video for 10 min.
DATA COLLECTED
The dataset includes three biological replicates. We collect over 10 worm videos and analyze defecation interval cycle (pBoc-pBoc time) and % of expulsion in 10 min recording. Statistical results are analyzed by ANOVA test.
The defecation cycle of C. elegans.
See reference.
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