C. elegans Behavior & Phenotyping
Embryo Viability
Reveal developmental effects of mutations and compound treatment by quantifying embryo viability.
APPLICATIONS
Mutations and compounds can affect animal viability. Embryo viability can reveal developmental effects of mutations and compound treatment.
ASSAY DESCRIPTION
Adult C. elegans are allowed to lay eggs on a plate for 2 hours. A set number of embryos are moved to a new plate. After 24 hours of incubation at 20℃, the number of unhatched embryos is counted.
DATA COLLECTED
The percentage of embryo lethality is calculated from the unhatched embryos divided by the total number of embryos plated. Three replicates are tested.
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