C. elegans Behavior & Phenotyping
Lipid Storage
The balance between lipid storage and utilization is an organism-wide process involving behavior, metabolism and signaling.
Lipid Storage Analysis. Wild-type C. elegans stained with RediStain Lipid Green show lipid accumulation in the intestine and epidermis.
APPLICATIONS
Lipid storage diseases such as Niemann-Pick are commonly studied in C. elegans.
ASSAY DESCRIPTION
Animals are stained with RediStain Lipid Green, a lipophilic fluorophore that tracks lipids inside C. elegans. When using Lipid Green, native lipid droplet morphology is preserved and fat content per body volume is quantified in individual worms.
DATA COLLECTED
Images of lipid droplets and quantification of fluorescence for 10 worms/condition are collected.
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