C. elegans Behavior & Phenotyping
Progeny Production
Reveal developmental effects of mutations and compound treatment by quantifying brood sizes.
Measuring brood size. NMX410 and NMX421 have a much lower brood size than N2 wild-type and several other samples.
APPLICATIONS
Mutations and compounds can affect animal viability and progeny production. Quantification of brood size can reveal developmental effects of mutations and compound treatment.
ASSAY DESCRIPTION
Single L4 C. elegans are incubated on a plate at 20℃ where they become adults and start laying eggs. The adult C. elegans are removed after 48 hours. Two days later the number of L3/L4 and adult worms are counted.
DATA COLLECTED
The number of progeny produced by each single animal is determined. Five biological replicates are tested.
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