How do I get rid of this pesky protein? The Hows and Whys of Protein Degradation
Being able to understand how a protein acts in specific tissues and times can be key for the general understanding of a protein’s function.
Gene Editing
How to identify a successful transgenic edit in C. elegans Part 1
In this blog, we will discuss key factors to consider when choosing a screening method, introduce various editing techniques, and discuss the pros & cons of various screening methods.
90 Mutations with 1 Gene: Functional Analysis of Variants Using C. elegans
We created over 90 point mutations in the STXBP1 gene via CRISPR. A map of these point mutations can be seen above. Clinical variants were selected from the ClinVar database, literature, the Gnomad database, clinical researchers, and the STXBP1 foundation.
PCR vs. HRMA for Genotyping: When to Use What?
We compare two screening methods – Polymerase Chair Reaction (PCR) and High Resolution Melt Analysis (HRMA), and examine when you would want to use each.
Fluorescent Tagging Capability Demonstration
At InVivo Biosystems, we have been working with fluorescent tags in C. elegans for over five years, and we have made over 600 fluorescent transgenic lines.
We Used CRISPR to Make 15 Point Mutations in 5 Genes. Here’s What We Learned.
You may or may not know that at InVivo Biosystems, we conduct a lot of grant funded research as well as offering custom models and assays to our clients. Much of our grant activity in the past few years has centered on creating and characterizing disease-associated variants and point mutations. We’ve created collaborations and partnerships …
We Used CRISPR to Make 15 Point Mutations in 5 Genes. Here’s What We Learned. Read More »
Zebrafish Injection Mix: Buy verses DIY, what are the real costs? [Infographic]
Make sure you understand all of the costs associated with DIY-ing a zebrafish CRISPR project verses outsourcing it to a team with years of experience.
Precise Knock-outs for Precise Research
Knockouts are the bread and butter of reverse genetic studies. They allow researchers to study the effects of the absence of a particular gene, protein segment, amino acid, or regulatory element, thereby enabling the researcher to determine their function. Many of the classically used and widely available knockout lines researchers rely on are not precise …
11 Steps to a Successful CRISPR Knock-in
Knock-ins are hard, for a long time people even said they were impossible, but we are nearing an 80% success rate for CRISPR knock-ins in zebrafish so we want to share with you what we believe to be the 11 steps necessary to have a successful knock-in project.
6 Key Steps to a Successful Zebrafish Knock-in [Infographic]
Successful zebrafish knock-ins are hard, we are not going to sugar coat it for you, but we at InVivo Biosystems are approaching an 80% success rate in our knock-ins and wanted to share with you what we believe to be the 6 key steps to having a successful knock-in project. Learn more about InVivo Biosystems’ …
6 Key Steps to a Successful Zebrafish Knock-in [Infographic] Read More »