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Gene Editing

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6 things that can go wrong while making your Zebrafish CRISPR Knock-In

With the advent of targeted nucleases like zinc-finger nucleases (ZFNs), TALE nucleases (TALENs), and clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas), zebrafish researchers were able to begin interrogating gene function and regions of interest with a precision that was previously out of reach. With these innovations, researchers could not only disrupt …

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To truly validate your sgRNAs, go In Vivo

CRISPR/Cas9 gene editing for Zebrafish is a rapidly growing molecular biology toolset with diverse applications, but is not without its technological barriers. Reagent sourcing, design and validation of cutting efficiency, and donor homology design are challenging, lengthy processes without guarantee of success even for highly skilled researchers. One way to enable researchers to initiate a …

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Zebrafish modeling for the clinic: Rapid in vivo functional testing of patient variants for clinical applications.

We use the zebrafish (Danio rerio) as an in vivo model to measure the functional effects of patient-derived genetic variation.  In this way, human genetic variants identified in the clinic are quantitatively and qualitatively connected to model animal phenotypes.  As a proof of concept study, we used precision gene editing and transient knockdown approaches targeting stxbp1a – the …

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CRISPR technologies enable humanized animal models to aid disease research

C. elegans as a model to evaluate the function of disease genes In 1998, the soil nematode Caenorhabditis elegans became the first multicellular organism of which the genome has been sequenced completely [1]. One surprising result of this approach was that ~65% of the human disease genes have a counterpart in the worm [2]. In …

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[Review] The Human Avatar

NemaMetrix is the market leader for early preclinical in-vivo testing using small animal models to gain a better understanding of the efficacy, mode of action, toxicity and potential targets for novel compounds. In this case study we outline how we used the animal model C. elegans to perform a set of specific experiments designed by our experts to provide rapid and reliable data based on our customer’s priorities. Using the data that we provided, our customer was able to apply for multiple patents in a timely manner.

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Using Zebrafish for Rapid Genetic Analysis

The introduction of engineered site-specific DNA endonucleases has brought precise genome editing in many model organisms into the realm of possibility. Thanks to precise and efficient genome editing in zebrafish using the CRISPR/Cas9 system, scientists at NemaMetrix are using zebrafish for rapid genetic analysis. Utilizing morphants and crispants you can…

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